常用缓冲溶液的配制方法1 1.甘氨酸-盐酸缓冲液（0.05mol/L）X毫升0.2 mol/L甘氨酸+Y毫升0.2 mol/L HCI，再加水稀释至200毫升pHXYpHXY2.05044.03.05011.42.45032.43.2508.22.65024.23.4506.42.85016.83.6505.0甘氨酸分子量=75.07, 0.2 mol/L甘氨酸溶液含15.01克/升2.邻苯二甲酸-盐酸缓冲液（0.05 mol/L）X毫升0.2mol/L邻苯二甲酸氢钾+ 0.2 mo>l/L HC1，再加 2K稀释到20毫升pH(20°C)XYpH(20C)XY2.254.0703.251.4702.453.9603.450.9902.653.2953.650.5972.852.6423.850.2633.052.022邻苯二甲酸氢钾分子量=204.23, 0.2 mol/L邻苯二甲酸氢溶液含40.85克/升3.磷酸氢二钠-柠檬酸缓冲液pH0.2mol/LNa2HPO4(毫升)0.1mol/L柠檬酸 （毫升）pH0.2mol/LNa2HPO4(毫升)0.1mol/L柠檬酸 （毫升）2.20.4010.605.210.729.282.41.2418.765.411.158.852.62.1817.825.611.608.402.83.1716.835.812.097.913.04.1115.896.012.637.373.24.9415.066.213.226.783.45.7014.306.413.856.153.66.4413.566.614.555.453.87.1012.906.815.454.554.07.7112.297.016.473.534.28.2811.727.217.392.614.48.8211.187.418.171.834.69.3510.657.618.731.274.89.8610.147.819.150.855.010.309.708.019.450.55Na2HPO4分子量=14.98, 0.2 mol/L溶液为28.40克/升。
Na2HPO4-2H2O分子量=178.05, 0.2 mol/L溶液含35.01 克/升C4H2O7 ・H2O分子量=210.14, 0.1 mol/L溶液为21.01 克/升4•柠檬酸-氢氧化钠-盐酸缓冲液pH钠离子浓度（mol/L）柠檬酸（克）C6H8O7 • HQ氢氧化钠（克）NaOH 97%盐酸（毫升）HCl （浓）最终体积（升）①2.20.2021084160103.10.2021083116103.30.2021083106104.30.202108345105.30.3524514468105.80.45285186105106.50.38266156126104 ①使用时可以每升中加入1克克酚，若最后pH值有变化，再用少量50%氢氧化钠溶液或浓盐酸调节，冰箱保存5②5.柠檬酸-柠檬酸钠缓冲液（0・1 mol/L）pH0.1 0.1 mol/L 柠檬酸（毫升）0.1 0.1 mol/L 柠檬酸钠（毫升）pH0.1 0.1 mol/L 柠檬酸（毫升）0.1 0.1 mol/L 柠檬酸钠（毫升）3.018.61.45.08.211.83.217.22.85.27.312.73.416.04.05.46.413.63.614.95.15.65.514.53.814.06.05.84.715.34.013.16.96.03.816.24.212.37.76.22.817.24.411.48.66.42.018.04.610.39.76.61.418.64.89.210.8柠檬酸C6H8O7 ・H2O：分子量210.14, 0.1 mol/L溶液为21.01克/升。
柠檬酸钠N°3 C6H5O7・2叩：分子量294.12, 0」皿血溶液为29.41克/毫升6.乙酸-乙E酸钠缓冲液（0・2 mol/L）0.2 0.2 mol/L0.3 0.3 mol/L0.2 0.2 mol/L0.3 0.3 mol/LpH（18 °CNaAcHAcpH（18 CNaAcHAc）（毫升）（毫升））（毫升）（毫升）2.60.759.254.85.904.103.81.208.805.07.003.004.01.808.205.27.902.104.22.657.355.48.601.404.43.706.305.69.100.904.64.905.105.89.400.60Na2Ac・3H2O分子量=136.09, 0.2 mol/L溶液为27.22克/升7.磷酸盐缓冲液（1）磷酸氢二钠-磷酸二氢钠缓冲液（0.2）pH0.2 0.2 mol/LNa2HPO4（毫升）0.3 0.3 mol/LNaH2PO4（毫升）pH0.2 0.2 mol/LNa2HPO4（毫升）0.3 0.3 mol/LNaH2PO4（毫升）5.88.092.07.061.039.05.910.090.07.167.033.06.012.387.77.272.028.06.115.085.07.377.023.06.218.581.57.481.019.06.322.577.57.584.016.06.426.573.57.687.013.06.531.568.57.789.510.56.637.562.57.891.58.56.743.556.57.993.07.06.849.551.08.094.75.3pHmol/L/15NaHPO,2 4(毫升)mol/L//15KHPO,2 4(毫升)pHmol/L//15NaHPO,2 4(毫升)mol/L//15KHPO 2(毫升)4.920.109.907.177.003.005.290.509.507.388.002.005.911.009.007.739.001.006.242.008.008.049.500.506.473.007.008.349.750.256.644.006.008.679.900.106.815.005.008.1810.0006.986.004.00Na2HPO4-2H2 O 分子量=178.05,Na2HPO4-2H2 0 分子量=358.22,Na2HPO4-2H2O 分子量=156.03, (2)磷酸氢二钠-磷酸二氢钾缓冲液Na2HPO4-2H2 O 分子量=178.05, 1/15M 溶液为 11.876克/升。
KH2PO4 分子量=136.09, 1/15M 溶液为9.078克 / 升6.955.045.00.2 mol/L溶液为35.61克/升0.2 mol/L溶液为71.6 4克/升0.2 mol/L溶液为31.21克/升1/15 mol/L)8.磷酸二氢钾-氢氧化钠缓冲液（0.05M）X毫升0.2M KPO + Y毫升0.2N NaO H加水稀释至29毫升pH(20C) Z； =F X （毫升）Y （毫升）pH(20C)X （毫升）Y （毫升）5.850.3727.052.9636.050.5707.253.5006.250.8607.453.9506.451.2607.654.2806.651.7807.854.5206.852.3658.054.6809•巴比妥钠-盐酸缓冲液（18°C）pH0.04M巴比妥 钠溶液0.2 V盐酸pH0.04M巴比 妥钠溶液（毫 升）0.2N盐酸（毫 升）6.810018.48.41005.217.010017.88.61003.827.210016.78.81002.527.410015.39.01001.657.610013.49.21001.137.810011.479.41000.708.01009.399.61000.358.21007.21100巴比妥钠盐分子量=206.18;0.04M溶液为8.25克/升10. Tris-盐酸缓冲液（0.05M, 25C）50毫升0.1M三羟甲基氨基甲烷（Tris）溶液与X毫升0.1N盐酸混匀后，加水稀释至100毫升。
pHX（毫 升）pHX（毫 升）7.1045.78.1026.27.2044.78.2022.97.3043.48.3019.97.4042.08.4017.27.5040.38.5014.77.6038.58.6012.47.7036.68.7010.37.8034.58.808.57.9032.08.907.08.0029.2三羟甲基氨基甲烷（Tris） H0CH2 CH20H CHOCH2 NH2分子量=121.14;1. 0. 1M溶液为12.114克/升Tris溶液可从空气中吸收二氧化碳，使用时注意将瓶盖严11^硼酸-硼砂缓冲M^^M硼酸根）pH0.05M硼砂（毫升）0.2M硼砂（毫 升）pH0.05M硼砂（毫升）0.2M硼酸（毫 升）7.41.09.08.23.56.57.61.58.58.44.55.57.82.08.08.76.04.08.03.07.09.08.02.0硼砂Na2B4O7^H2O，分子量=381.43;0.05M溶液（=0.2M硼酸根）含19.07克/升 硼酸H2BO3,分子量=61.84,0.2M溶液为12.37克/升硼砂易2失去3 结晶水，必须在带塞的瓶中保存。
11.硼酸-硼砂缓冲液（0.2M硼酸根）pH0.05M硼砂（毫升）0.2M硼酸（毫 升）pH0.05M硼砂（毫升）0.2M硼酸（毫 升）7.41.09.08.23.56.57.61.58.58.44.55.57.82.08.08.76.04.08.03.07.09.08.02.0硼砂Na2B4O7^10H2O,分子量=381.43;0.05M溶液（=0.2M硼酸根）含 19.07克/升 硼酸H2BO3，分子量=61.84, 0.2M溶液为12.37克/升硼砂 2 易3失去结晶水，必须在带塞的瓶中保存12•甘氨酸-氢氧化钠缓冲液（0.05M）X毫升0.2M甘氨酸+Y毫升0.2NnaO H加水稀释至200毫升pHXYpHXY8.6504.09.65022.48.8506.09.85027.29.0508.810.05032.09.25012.010.45038.69.45016.810.65045.5甘氨酸分子量=75.07;0.2M溶液含15.01克/升13.硼砂-氢氧化钠缓冲液（0.05M硼酸根）X毫升0.05M硼砂+Y毫升0.2NNaO H加水稀释至200毫升pHXYpHXY9.3506.09.85034.09.45011.010.05043.09.65023.010.15046.0硼砂 N a2B4O7^10H2O,分子量=381.43;0.05M 溶液为 19.07 克/升。
14.碳酸钠-碳酸氢钠缓冲液（0.1M）Ca2+、Mg2+存在时不得使用pH毫升毫升20 °C37 C9.168.77199.409.12289.519.40379.789.50469.909.725510.149.906410.2810.087310.5310.288210.8310.5791Na2CO2^10H2 O 分子量=286.2;0.1M 溶液为28.62克/升N2HCO3分子量=84.0;0.1M溶液为8.40克/升15. “PBS”缓冲液PH7.67.47.27.0h2o100010001000100mlNaCl8.58.58.58.5gNa2HPO42.22.22.22.2gNaH2PO40.10.20.30.4g实验室中常用酸碱的比重和浓度名称分子式分子量比重面分浓度 % （W/W）当量浓度（粗略）N配1升1N溶 液所需毫 升数盐酸HCl36.471.1937.212.0841.1835.411.81.1020.06.0硫酸H SO,2 498.091.8495.636.0281.1824.86.0硝酸hno263.021.4270.9816.0631.4065.314.51.2032.366.1冰乙酸ch3cooh60.051.0599.517.459乙酸CH；COOH98.06366.0磷酸H2PO435.051.7185.015,30,45（依反应而 定）67（以 15N计）氨水NH OH40.9015670.90427.014.3700.9125.013.40.9610.05.6氢氧化 钠溶液NaOH40.01.550.01953元素的原子量表（录自1997年国际原子量表，并全部取四位有效数字）兀素符号原子量原子序数Ac Ag Al Am Ar As At Au B Ba Be Bi Bk Br C Ca Cd Ce Cf Cl Cm Co Cr Cs Cu Dy Er Es Eu F Fe Fm Fr Ga Gd Ge H He Hf Hg HoI In Ir K Kr La Li227.0107.926.98[243]39.9574.92[210]197.010.81137.39.012209.0[247]79.9012.0140.08112.4140.1[251]35.45[247]58.9352.00132.963.55162.5167.3[254]152.019.0055.85[257][223]69.72157.372.591.0084.003178.5200.6164.9126.9114.8192.239.1083.80138.96.941894713951833857955648397350204858981796272455296668996392610087316432127280675349771936573锢錶钾氟精锂e镑钥镁猛钳氮钠铳枚M镰错B氧娥磷镁铅耙<针错钳环镭御铢错氮钉硫铳铳硒硅侈锡總袒