b1b211b11b222b12b2各组间相互交叉，因此，析因实验设计也称为交叉分组设计。各组间相互交叉，因此，析因实验设计也称为交叉分组设计。X第一组第一组第二组第二组第三组第三组第四组第四组合计合计（一般疗（一般疗法）法）（一般疗法（一般疗法+甲药）甲药）（一般疗法（一般疗法+乙药）乙药）（一般疗法（一般疗法+甲甲药药+乙药）乙药）0.81.30.92.15.10.91.21.12.25.40.71.11.02.04.8X2.43.63.06.315.30.81.21.02.11.28X21.944.343.0213.2522.55 乙药乙药合计合计不用不用用用甲药甲药不用不用2.43.05.4用用3.66.39.9合计合计6.09.315.3变异来源lMSF总处理间32.96甲 药11.691.69169*乙 药10.910.9191*交互作用10.360.3636*误 差80.080.01总 变 异113.04测定测定次数次数经高锰酸盐处理经高锰酸盐处理未经高锰酸盐处理未经高锰酸盐处理测定测定合计合计0.25g1.00g0.25g1.00g样本样本样本样本样本样本样本样本127.224.639.538.6129.9223.224.243.139.5130.0324.822.245.233.0125.2合计合计75.271.0127.8111.1385.1样本重量样本重量高锰酸盐高锰酸盐行合计行合计处理处理未处理未处理0.25g75.2127.8203.01.00g71.0111.1182.1列合计列合计146.2238.9385.1变异来源变异来源lMSF总变异总变异818.3711测定间变异测定间变异3.7621.88处理间变异处理间变异(765.11)(3)高锰酸盐高锰酸盐716.111716.1187.54*样本重量样本重量36.40136.404.45变互作用变互作用13.02113.021.59误差误差49.0868.18AB1B2C1C2C1C2A1A1B1C1A1B1C2A1B2C1A1B2C2A2A2B1C1A2B1C2A2B2C1A2B2C2A1B1C1A1B1C2A1B2C1A1B2C2A2B1C1A2B1C2A2B2C1A2B2C20.540.740.430.520.690.760.610.550.550.610.500.490.540.730.570.480.740.770.580.490.700.630.670.540.710.600.650.490.610.610.710.490.620.580.510.490.730.840.670.420.580.790.570.610.700.620.600.600.510.570.680.590.590.670.630.640.560.720.660.620.610.660.660.48合计合计4.815.384.584.295.175.525.124.20B1B2合计合计C1C2C1C2A14.815.384.584.2919.06（A1）A25.175.525.124.2020.01（B2）C1B1C2C1B2C2A110.198.87A210.699.32B1C1B2B1C2B2A19.399.67A210.299.72A1C1A2A1C2A2B19.9810.90B29.708.49变异来源变异来源SSMSF总变异总变异0.527563总处理间总处理间0.21237性别间性别间0.014110.01412.158大豆间大豆间0.113110.113020.196*玉米间玉米间0.001310.00130.232一级交互作用一级交互作用AB0.00001AC0.011310.01132.018BC0.070910.070912.661*二级交互作用二级交互作用0.001610.00160.286误差误差0.3152560.0056因因 素素水水 平平基础液（基础液（A）缓冲剂（缓冲剂（A1）蒸馏水（蒸馏水（A2）自来水（自来水（A3）血清种类血清种类（B）免血清（免血清（B1）胎盘血清胎盘血清（B2）血清浓度血清浓度（C）5%（C1）8%（C2）有添加剂有添加剂的基础液的基础液（A）血清种类（血清种类（B）免血清免血清（B1）胎盘血清胎盘血清（B2）血清浓度血清浓度（C）血清浓度血清浓度（C）5%（C1）8%（C2）5%（C1）8%（C2）缓冲剂（缓冲剂（A1）14261260604110811831599108188620001410487831161224166241159蒸馏水（蒸馏水（A2）6848758671115143022507716981165187140379120221962370559自来水（自来水（A3）118212201243128315121095111511421450170041667713852372533534有添加剂的基础液（A）血清种类（B）免血清（B1）胎盘血清（B2）血清浓度（C）血清浓度（C）5%（C1）8%（C2）5%（C1）8%（C2）缓冲剂（A1）14261260604110811831599108188620001410487831161224166241159蒸馏水（A2）6848758671115143022507716981165187140379120221962370559自来水（A3）11821220124312831512109511151142有添加剂有基础液（A）血清种类（B）免血清胎盘血清（B2）基础液血清浓度（C）血清浓度（C）合计5%（C1）8%（C2）X5%（C1）8%（C2）缓冲液（A1）622166852796398419686蒸馏水（A2）530169582411316317833自来水（A3）552963873307363618859BC（合计）170512003085141078356378（总计）X2缓冲剂10031591196955721599224046502蒸馏水79584651317821016454392668591自来水77039931120380932454193693738 79505154 X缓冲剂1555.251671.25699.00996.00蒸馏水1326.251739.50602.75790.75自来水1382.251596.75826.75909.001174.54（总均数）S2 缓冲剂118766.25265750.2568506.0026146.00蒸馏水31104.92358256.3364069.5855816.25自来水20510.92335122.25170452.25129538.00AB合计 AC合计免血清胎盘血清5%8%缓冲剂129066780901710669蒸馏水122595574771210121自来水119166943883610023合 计37081192972556530813有添加剂有基础液（A）血清种类（B）免血清胎盘血清（B2）基础液血清浓度（C）血清浓度（C）合计5%（C1）8%（C2）X5%（C1）8%（C2）缓冲液（A1）622166852796398419686蒸馏水（A2）530169582411316317833自来水（A3）552963873307363618859BC（合计）170512003085141078356378（总计）X2缓冲剂10031591196955721599224046502蒸馏水79584651317821016454392668591自来水77039931120380932454193693738 X缓冲剂1555.251671.25699.00996.00蒸馏水1326.251739.50602.75790.75自来水1382.251596.75826.75909.001174.54（总均数）S2 缓冲剂118766.25265750.2568506.0026146.00蒸馏水31104.92358256.3364069.5855816.25自来水20510.92335122.25170452.25129538.00AB合计 AC合计免血清胎盘血清5%8%缓冲剂129066780901710669蒸馏水122595574771210121自来水119166943883610023合 计37081192972556530813变异来源SSMSF总变异13286843.91747主效应基础液（A）107712.792253856.400.336血清种类（B）6588972.00016588972.0041.095*血液浓度（C）573781.3381573781.333.579交互作用AB95267.375247633.690.297AC47553.292223776.650.148BC10502.084110502.080.066ABC90938.041245469.020.284剩余（即误差）577211736160.33658无添加剂的基础液（A）血清种类（B）免血清胎盘血清血清浓度（C）血清浓度（C）5%8%5%8%缓冲剂64811448305781246187785366913981671441643909184510301002蒸馏水1763144792093312411883709102413811896848109224211926574742自来水5801789112668510261215117654610261434128059583016511212566变异不源SSMSF总变异1038749631347主效应基础液（A）679967.3752339983.694.977*血清种类（B）4184873.52114184873.5261.361*血清浓度（C）238713.021123713.023.494交互作用AB705473.0422352736.525.164*AC107005.542253502.770.783BC108922.68711089922.6915.955*ABC922307.3752461153.696.751*剩余（即误差）2459233.7503668312.05各小组合计各小组均数缓冲剂蒸馏水自来水缓冲剂蒸馏水自来水兔血清5%4201680634621050.251701.50865.508%6537715260891634.251788.001522.25胎盘血清5%315430514794788.50762.751198.508%289237912392723.00947.75598.00组数23r0.01,302.893.04r0.01,402.863.01r0.01,303.894.06r0.01,403.823.99组数23r0.01,40373.75393.36r0.01,40499.21521.42X X X X X X X X X X X XBC兔血清的相差BC兔血清基础液-865.50-1050.50基础液1522.25-1634.25蒸馏水1701.50836.00*651.25*蒸馏水1788.00265.75153.75缓冲剂1050.25184.75缓冲剂1634.25112.00自来水865.50自来水1522.25BC胎盘血清浓度5%BC胎盘血清浓度8%基础液-762.75788.50基础液-598.00-723.00自来水1198.50435.75*410.00*自来水947.50349.75224.75缓冲液788.5025.75缓冲液723.00125.00蒸馏水762.75蒸馏水598.00基础液浓度兔血清胎盘血清相差缓冲剂5%1050.25788.50261.758%1634.25723.00911.25*蒸馏水5%1701.50762.75938.75*8%1788.00947.75840.25*自来水5%865.501198.50333.008%1522.25598.00924.25*基础液血清种类浓度8%浓度5%相差缓冲剂兔血清1634.251050.25584.00*蒸馏水兔血清1788.001701.5086.50自来水兔血清1522.25865.50656.75*缓冲剂胎盘血清723.00788.50-65.50蒸馏水胎盘血清947.75762.75185.00自来水胎盘血清598.001198.50600.50*